Rapid chromatographic purification of urinary steroid glucuronates for determination of aldosterone secretion rate.
J Chromatogr
; 317: 367-76, 1984 Dec 28.
Article
en En
| MEDLINE
| ID: mdl-6530444
Aldosterone secretion rate (ASR) is utilized as a rapid screening procedure to detect subtle forms of hypertension. A rapid and robust chromatographic method has been developed, based on easily prepared, rigid matrices, which permit the flow of urine by suction through sequential purification columns. Both major metabolites, aldosterone glucuronate and tetrahydroaldosterone glucuronate, are isolated in ca. 75% yield. They are hydrolyzed and quantitated by high-performance liquid chromatography-radioimmunoassay (HPLC-RIA) or, for bulk preparation, further purified by preparative chromatofocusing. These two polar conjugates are isolated in nearly pure form by HPLC on a C2 column with a two-step gradient. An ASR determination can be completed in one to two working days; preparative-scale work takes somewhat longer.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aldosterona
/
Glucuronatos
Límite:
Humans
Idioma:
En
Revista:
J Chromatogr
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Países Bajos