Urinary malondialdehyde as an indicator of lipid peroxidation in the diet and in the tissues.
Lipids
; 19(11): 836-43, 1984 Nov.
Article
en En
| MEDLINE
| ID: mdl-6521608
Although malondialdehyde (MDA) is extensively metabolized to CO2, small amounts are nevertheless excreted in an acid-hydrolyzable form in rat urine. In this study, urinary MDA was evaluated as an indicator of lipid peroxidation in the diet and in the tissues. MDA was released from its bound form(s) in urine by acid treatment and determined as the TBA-MA derivative by HPLC. MDA excretion by the rat was found to be responsive to oral administration of the Na enol salt and to peroxidation of dietary lipids. Urinary MDA also increased in response to the increased lipid peroxidation in vivo produced by vitamin E deficiency and by administration of iron nitrilotriacetate. Chronic feeding of a diet containing cod liver oil led to increases in MDA excretion which were not completely eliminated by fasting or feeding a peroxide-free diet, indicating that there was increased lipid peroxidation in vivo. MDA excretion was not responsive to Se deficiency or CCl4 administration. DPPD, a biologically active antioxidant, but not BHA, a non-biologically active antioxidant, prevented the increase in MDA excretion in vitamin E deficient animals. The results indicate that MDA excretion can serve as an indicator of the extent of lipid peroxidation in the diet and, under conditions which preclude a dietary effect, as an index of lipid peroxidation in vivo.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Dieta
/
Peróxidos Lipídicos
/
Malonatos
/
Malondialdehído
Límite:
Animals
Idioma:
En
Revista:
Lipids
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Estados Unidos