Biosynthesis and regulation of the peroxisomal methanol oxidase from the methylotrophic yeast Hansenula polymorpha.
Mol Gen Genet
; 194(3): 489-93, 1984.
Article
en En
| MEDLINE
| ID: mdl-6377014
The biosynthesis of methanol oxidase, a peroxisomal enzyme in the methanol-utilizing yeast Hansenula polymorpha, was studied in vitro. Translation of Hansenula mRNA in a rabbit reticulocyte lysate yields methanol oxidase protein in high amounts. The apparent molecular mass of the protein was found to be identical to the subunit of the functional multimeric enzyme, which indicates the absence of an N-terminal extension typical of most transported proteins. The regulation of methanol oxidase by glucose repression and depression as well as by induction of methanol was shown to be controlled at the level of transcription. Two mutants of Hansenula polymorpha, unable to grow on methanol as a carbon and energy source were shown to be affected in methanol oxidase synthesis.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pichia
/
Ascomicetos
/
Proteínas Fúngicas
/
Regulación de la Expresión Génica
/
Oxidorreductasas de Alcohol
Idioma:
En
Revista:
Mol Gen Genet
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Alemania