An immunoassay for human D dimer using monoclonal antibodies.

Rylatt, D B; Blake, A S; Cottis, L E; Massingham, D A; Fletcher, W A; Masci, P P; Whitaker, A N; Elms, M; Bunce, I; Webber, A J

Rylatt, D B; Blake, A S; Cottis, L E; Massingham, D A; Fletcher, W A; Masci, P P; Whitaker, A N; Elms, M; Bunce, I; Webber, A J.

Thromb Res ; 31(6): 767-78, 1983 Sep 15.

Article en En | MEDLINE | ID: mdl-6359576

RESUMEN

Monoclonal antibodies (MAb) were raised against human D dimer. The hybridomas were screened with a solid phase enzyme immunoassay against D dimer and fibrinogen degradation products. Among the panel of MAb identified, two distinct patterns emerged; the majority belonging to a panspecific class reacting against epitopes present on both D dimer and fibrinogen degradation product Dcate and a monospecific class reacting with determinants apparently present only on D dimer. A number of MAb were further characterised for their ability to specifically capture antigen in a solid phase enzyme immunoassay and assays were developed which have a sensitivity of 10 ng/ml for D dimer or crosslinked fibrin derivatives and may be suitable for detection of crosslinked derivatives in serum and plasma samples in a clinical situation.

Asunto(s)

Anticuerpos Monoclonales/inmunología; Productos de Degradación de Fibrina-Fibrinógeno/análisis; Animales; Especificidad de Anticuerpos; Reacciones Cruzadas; Coagulación Intravascular Diseminada/diagnóstico; Productos de Degradación de Fibrina-Fibrinógeno/inmunología; Fibrinólisis; Humanos; Técnicas para Inmunoenzimas; Ratones

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Productos de Degradación de Fibrina-Fibrinógeno / Anticuerpos Monoclonales Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Thromb Res Año: 1983 Tipo del documento: Article Pais de publicación: Estados Unidos

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