Evidence that levels of malate dehydrogenase and fumarase are increased by cAMP in rat myotubes.
Am J Physiol
; 247(1 Pt 1): C33-8, 1984 Jul.
Article
en En
| MEDLINE
| ID: mdl-6331185
We have investigated the potential role of adenosine 3',5'-cyclic monophosphate (cAMP) in controlling levels of enzymes of energy metabolism in primary cultures of rat skeletal muscle cells. Incubating myotubes with cholera toxin or forskolin (2 persistent activators of adenylate cyclase) significantly increased the levels of two enzymes of oxidative metabolism, fumarase and malate dehydrogenase. These enzymes were also increased (1.5- to 2.0-fold) by phosphodiesterase inhibitors (caffeine, theophylline, theobromine, 3-isobutyl-1-methylxanthine, papaverine, MJ 1988, Ro 20-1724, or SQ 20009) and the cAMP derivatives: 8-bromo-cAMP or dibutyryl cAMP. In contrast two enzymes of glycolytic metabolism, lactate dehydrogenase and pyruvate kinase, were not consistently affected by these agents. The results presented provide strong evidence that an increase in cAMP can lead to an increase in certain enzymes of oxidative energy metabolism.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
AMP Cíclico
/
Fumarato Hidratasa
/
Malato Deshidrogenasa
/
Músculos
Límite:
Animals
Idioma:
En
Revista:
Am J Physiol
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Estados Unidos