Binding of simian virus 40 a protein to DNA with deletions at the origin of replication.
J Virol
; 49(1): 9-13, 1984 Jan.
Article
en En
| MEDLINE
| ID: mdl-6317897
Previous studies with wild-type simian virus 40 DNA have shown that the sequence 5'-GAGGC-3' directs the binding of A protein (T antigen). The functional origin of replication contains four recognition pentanucleotides each of which is separated by a single base pair and arranged a two pairs of direct repetitions that are inverted relative to each other. Analysis of A protein binding to a series of nonviable mutants progressively deleting these contact sites leads to the following conclusions: (i) stable binding of subunits of A protein to three origin pentanucleotides is not sufficient for the initiation of DNA replication, (ii) the stability of DNA binding depends on interactions between bound protein subunits, and (iii) a single pentanucleotide is sufficient to bind and orient a subunit of A protein.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
Virus 40 de los Simios
/
Proteínas de Unión al ADN
Idioma:
En
Revista:
J Virol
Año:
1984
Tipo del documento:
Article
Pais de publicación:
Estados Unidos