Cloning of bacterial DNA replication genes in bacteriophage lambda.
Mol Gen Genet
; 187(3): 501-9, 1982.
Article
en En
| MEDLINE
| ID: mdl-6294475
Recombinant lambda phages containing the genes for dnaZ protein (the gamma subunit of DNA polymerse III holoenzyme), primase (dnaG protein) and dnaC protein from Escherichia coli and Salmonella typhimurium were isolated. Each gene cloned from S. typhimurium has extensive DNA sequence homology to the corresponding E. coli gene. Clones selected by complementation of a dnaA temperature-sensitive mutant appear similar to other isolated suppressors of dnaA (Projan and Wechsler 1981). Derivatives of each cloned fragment suitable for overproduction of the protein were constructed. Of those tested, only the phage containing the E. coli dnaZ gene resulted in significant overproduction.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN Bacteriano
/
Clonación Molecular
/
Bacteriófago lambda
/
Replicación del ADN
/
Escherichia coli
/
Genes
/
Genes Bacterianos
Idioma:
En
Revista:
Mol Gen Genet
Año:
1982
Tipo del documento:
Article
Pais de publicación:
Alemania