Effects of sodium and bicarbonate ions on gamma-aminobutyric acid receptor binding in synaptic membranes of rat brain.
J Neurochem
; 37(2): 418-21, 1981 Aug.
Article
en En
| MEDLINE
| ID: mdl-6267202
Crude synaptic membranes treated with Triton X-100 (TX) bound gamma-aminobutyric acid (GABA) to two classes of receptor site in Na+-free 10 mM-Tris-sulfate buffer (pH 7.4), but to only a single class of receptor site in 10 mM Tris-sulfate buffer (pH 7.4), containing 150 mM-NaCl. The high-affinity receptor site in TX membranes was specifically masked in the presence of Na+. However, TX membranes incubated in Krebs-Ringer bicarbonate solution (pH 7.4) bound GABA to two classes of receptor site despite the presence of Na+. It was found that addition of bicarbonate ions to the Na+-containing 10 mM-Tris-sulfate buffer (pH 7.4) could restore that high-affinity class of GABA receptors, rendering both classes detectable. This finding suggests that both Na+ and HCO-3 may have a regulatory function on GABA binding to the receptor.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Sodio
/
Membranas Sinápticas
/
Bicarbonatos
/
Encéfalo
/
Receptores de Superficie Celular
/
Ácido gamma-Aminobutírico
Límite:
Animals
Idioma:
En
Revista:
J Neurochem
Año:
1981
Tipo del documento:
Article
Pais de publicación:
Reino Unido