Gas chromatography chemical ionization mass spectrometric analysis of diminazene in plasma.
Biomed Mass Spectrom
; 5(1): 72-5, 1978 Jan.
Article
en En
| MEDLINE
| ID: mdl-623896
A quantitative and sensitive method was developed for the determination of diminazene in plasma. The assay involves the reduction of diminazene to 4-aminobenzamidine and 4-hydrazinobenzamidine. The latter is further reduced to give an additional mole of 4-aminobenzamidine which is extracted, acetylated and condensed with hexafluoroacetylacetone to form a volatile derivative that is subsequently analyzed by gas chromatography chemical ionization mass spectrometry. 4-Aminobenzylamidine was synthesized and used as an internal standard. The method is reproducible and its sensitivity limit using 1 ml of plasma is 0.1 microgram diminazene ml-1. This sensitivity limit is sufficient to detect plasma levels in cattle following therapeutic doses of the drug.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Diminazeno
/
Amidinas
Límite:
Animals
Idioma:
En
Revista:
Biomed Mass Spectrom
Año:
1978
Tipo del documento:
Article
Pais de publicación:
Reino Unido