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Purification, sequencing and synthesis of natriuretic and vasoactive rat atrial peptide.
Nature ; 309(5970): 717-9, 1984.
Article en En | MEDLINE | ID: mdl-6233494
Mammalian atria contain potent natriuretic and diuretic substances which exist in high- and low-molecular-weight forms and which appear to be associated with atrium-specific granules. The natriuretic effect of atrial extract is largely accountable for by its renal haemodynamic effects; atrial extracts also antagonize hormone- and non-hormone-induced contraction of the isolated rabbit aorta and isolated rat kidney vasculature. We have completely purified a low-molecular-weight natriuretic and vasoactive substance from rat atria and characterized it as a 24-amino acid peptide. Synthetic peptide, produced by solid-phase synthesis, mimics biological effects of crude atrial extract and purified peptide; its activity is enhanced by slow oxidation, suggesting a disulphide (Cys 4-Cys 20) configuration for the native peptide. If secreted into blood, this atrial natriuretic peptide (' auriculin B') could be a novel peptide hormone of considerable importance to renal and cardiovascular homeostasis.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Riñón / Proteínas Musculares / Natriuresis Límite: Animals Idioma: En Revista: Nature Año: 1984 Tipo del documento: Article Pais de publicación: Reino Unido
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas / Riñón / Proteínas Musculares / Natriuresis Límite: Animals Idioma: En Revista: Nature Año: 1984 Tipo del documento: Article Pais de publicación: Reino Unido