Development and characteristics of a subline of Ehrlich ascites carcinoma cells persistently resistant to 5-fluoro-2'-deoxyuridine.
Acta Biochim Pol
; 30(2): 185-91, 1983.
Article
en En
| MEDLINE
| ID: mdl-6223475
A subline of Ehrlich ascites carcinoma (EAC) cells resistant to 5-fluoro-2'-deoxy-uridine (FdUrd) was developed by continuous exposure to progressively increasing concentrations of the drug (35-75 mg/kg per day) during 15 passages through mice. Since then, the EAC cells have been retransplanted more than 80 times through drug-untreated mice and continue to be resistant. After adaptation to growth in suspension culture the drug-adapted cells were 1000 times more resistant to FdUrd in comparison with parental ones, and remained near-tetraploid with doubling time longer than in parental line. The activity of thymidine kinase was deeply depressed (100-fold) whereas that of thymidylate synthetase several-fold increased in the resistant EAC cells, both grown in vivo and in vitro.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Carcinoma de Ehrlich
/
Floxuridina
Límite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Acta Biochim Pol
Año:
1983
Tipo del documento:
Article
Pais de publicación:
Polonia