RNA synthesis in primary cultures of adult rat hepatocytes.
In Vitro
; 19(4): 299-306, 1983 Apr.
Article
en En
| MEDLINE
| ID: mdl-6189778
The ability of hepatocyte monolayers to synthesize RNA was investigated by measuring [3H]orotic acid incorporation into RNA and the total nuclear RNA polymerase activity as a function of the time in culture. The results demonstrate that primary cultures of hepatocytes maintained in a chemically defined serum- and hormone-free medium are able to synthesize RNA actively. This ability increases within the first 2 d of culture, despite the concomitant decrease in [3H]orotic acid uptake, and decreases only after 3 d. Factors such as serum, insulin, and dexamethasone, known to improve maintenance of functional hepatocytes, markedly stimulate the uptake of labeled precursor without apparently affecting the rate of RNA synthesis by cultured cells. It is suggested that the culture of adult rat hepatocytes provides a useful experimental model for the studies of hormonal regulation of transcription in liver.
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Asunto principal:
Transcripción Genética
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Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
In Vitro
Año:
1983
Tipo del documento:
Article
Pais de publicación:
Estados Unidos