2'5' oligo(A) polymerase activity in serum of mice infected with EMC virus or treated with interferon.
Nature
; 285(5765): 485-8, 1980 Jun 12.
Article
en En
| MEDLINE
| ID: mdl-6157092
Interferon-treated cells show an increase in two double-stranded RNA (dsRNA)-dependent enzymatic activities involving an oligoadenylate polymerase and a protein kinase (ref. 1 and refs therein). The polymerase converts ATP into a series of oligonucleotides characterized by 2'5'-phosphodiester bonds, designated 2'5'-oligo(A) or 2-5A (ref. 1). These oligonucleotides activate an endoribonuclease that degrades RNA in extracts of control and interferon-treated cells. These observations have been made in tissue culture cells and no informatin is yet available on these enzymatic activities in animals with elevated interferon levels. We report here on 2-5A synthesis in tissue homogenates and serum of mice infected with encephalomyocarditis virus (EMCV); this virus induces interferon synthesis when injected intraperitoneally into mice. Significant synthesis of 2-5A was detected in extracts of spleen and lungs, but also, surprisingly, in the serum of these mice. Subsequent experiments showed synthesis of 2-5A in serum of mice treated with the interferon inducer poly(I) x poly(C) (ref. 3) or with mouse fibroblast interferon.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Polinucleótido Ligasas
/
Interferones
/
Infecciones por Enterovirus
Límite:
Animals
Idioma:
En
Revista:
Nature
Año:
1980
Tipo del documento:
Article
Pais de publicación:
Reino Unido