Biochemical properties and immunogenicity of L-phenylalanine ammonia-lyase: effects on tumor-bearing mice.
Cancer Treat Rep
; 63(6): 1063-8, 1979 Jun.
Article
en En
| MEDLINE
| ID: mdl-466645
L-Phenylalanine ammonia-lyase (PAL) from yeast was used to deplete plasma L-phenylalanine and L-tyrosine in an attempt to achieve inhibition of tumor growth in mice. Plasma L-phenylalanine and L-tyrosine were reduced to nondetectable levels when circulating PAL activity was maintained at greater than or equal to 0.06 unit/ml. Repeated administration resulted in the appearance of anti-PAL antibodies. A radioimmunoassay based on the method of Farr was developed to determine quantitatively the presence of anti-PAL. Sublethal total-body irradiation temporarily suppressed the immunologic response of the host. Long-term specific immunosuppression to PAL was achieved with cyclophosphamide (CPA). A single dose of 180 mg/kg of CPA administered ip to mice 24 hours before, simultaneously with, or 24 hours after 100 units/kg of PAL induced tolerance for 450 days (20 injections of enzyme). The plasma half-life of PAL in CPA-treated mice remained essentially the same as that found after a single injection (25 hours), and anti-PAL probably will require specific immunosuppression of the host to repeated injections of the enzyme.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fenilanina Amoníaco-Liasa
/
Amoníaco-Liasas
/
Neoplasias Experimentales
Límite:
Animals
Idioma:
En
Revista:
Cancer Treat Rep
Año:
1979
Tipo del documento:
Article
Pais de publicación:
Estados Unidos