Effect of interferon on chemiluminescence and hydroxyl radical production in murine macrophages stimulated by PMA.
Immunology
; 56(3): 533-41, 1985 Nov.
Article
en En
| MEDLINE
| ID: mdl-4077098
Considerably augmented chemiluminescence (CL) occurred when murine peritoneal resident macrophages (MPs), pretreated with murine interferon (MuIFN)-alpha within 24 hr, were stimulated by 4-beta-phorbol, 12-beta-myristate, 13-beta-acetate (PMA). Augmentation of CL generation ceased when incubation in the presence of MuIFN was continued for 48 hr. As 12 hr preincubation with MuIFN procured optimal CL generation, the various reactive oxygen species (OH, O2-., H2O2) were measured at that point. The hydroxyl radical (OH.) level in MuIFN-treated MPs was 19.44 times higher than in MuIFN-untreated MPs. However, the levels of O2-. and H2O2 generation were the same in both MuIFN-treated and untreated MPs. Moreover, by using the inhibitors lipoxygenase and cyclo-oxygenase, we established clearly that CL and OH. generation in MuIFN-treated MPs is due to the lipoxygenase pathway of arachidonic acid metabolism.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Forboles
/
Acetato de Tetradecanoilforbol
/
Interferón Tipo I
/
Mediciones Luminiscentes
/
Macrófagos
Límite:
Animals
Idioma:
En
Revista:
Immunology
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Reino Unido