The effect of transferrin saturation on the estimation of erythropoietin by the mouse spleen cell microassay.

Lappin, T R; Elder, G E; McKibbin, S H; McNamee, P T; McGeown, M G; Bridges, J M

Lappin, T R; Elder, G E; McKibbin, S H; McNamee, P T; McGeown, M G; Bridges, J M.

Exp Hematol ; 13(10): 1007-13, 1985 Nov.

Article en En | MEDLINE | ID: mdl-4054239

RESUMEN

The effect of transferrin from various sources and the degree of saturation with iron on the stimulation of DNA synthesis by erythropoietin (Epo) has been investigated. Mouse, human, and bovine transferrins saturated with iron caused an increase in thymidine incorporation both in the absence and presence of Epo. In contrast, exogenous human and bovine apotransferrin resulted in significantly decreased incorporation of the tracer. The iron saturation of serum alters its apparent erythropoietic activity. This transferrin saturation effect may be overcome by a simple modification involving the addition of iron to the culture medium.

Asunto(s)

Eritropoyetina/análisis; Linfocitos/metabolismo; Transferrina/farmacología; Animales; Bovinos; Replicación del ADN/efectos de los fármacos; Eritropoyetina/farmacología; Eritropoyetina/orina; Femenino; Humanos; Hierro/metabolismo; Cinética; Linfocitos/efectos de los fármacos; Métodos; Ratones; Ratones Endogámicos C57BL; Microquímica; Especificidad de la Especie; Bazo/metabolismo; Transferrina/metabolismo

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transferrina / Linfocitos / Eritropoyetina Límite: Animals / Female / Humans Idioma: En Revista: Exp Hematol Año: 1985 Tipo del documento: Article Pais de publicación: Países Bajos

Buscar en Google

Añadir a Mi BVS

Imprimir

XML

PubMed Links