Deoxyribonucleic acid turnover in immature neurons of the rat cerebral cortex.
Neurosci Lett
; 58(3): 311-4, 1985 Aug 05.
Article
en En
| MEDLINE
| ID: mdl-4047491
To test for metabolic deoxyribonucleic acid (DNA) turnover in differentiating neurons, [methyl-3H]thymidine was injected into the lateral cerebral ventricles of newly born rats, and after 6, 24 and 96 h, neuronal nuclei were prepared from the immature cerebral cortex. Enzymatic treatment converted virtually all of the DNA into soluble deoxynucleosides which were fractionated by high-performance liquid chromatography for determination of specific activity. The specific activity of thymidine was found to decline rapidly with time. The rate of this loss correlated with the radioactivity initially incorporated into the DNA. This suggested that DNA was being replaced by DNA repair as a consequence of radiation damage, rather than by spontaneous metabolic DNA turnover.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Corteza Cerebral
/
Reparación del ADN
Límite:
Animals
Idioma:
En
Revista:
Neurosci Lett
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Irlanda