Envelope glycoproteins of Rauscher murine leukemia virus: isolation and chemical characterization.
Biochemistry
; 16(4): 710-7, 1977 Feb 22.
Article
en En
| MEDLINE
| ID: mdl-402147
The envelope glycoproteins (designated gp70 and gp45) of the Rauscher strain of murine leukemia virus were solubilized by osmotic shock and freeze-thawing in chaotropic solutions. The viral glycoproteins were then purified by phosphocellulose chromatography and gel permeation chromatography on Bio-Gel A-1.5m. Yields by this procedure were 6.2% for gp70 and 1.3% for gp45 on a protein input basis. The apparent molecular weights were respectively 67 500 and 47 500 with a polypeptide chain molecular weight of approximately 45 000 for both glycoproteins. Amino acid analysis showed a high degree of similarity for both components, with some differences subject to further evaluation. The total carbohydrate content was approximately 32% for gp70 and 6-9% for gp45. In keeping with the amino acid compositional similarity suggesting relationships, alanine was found to ba the amino-terminal amino acid of both glycoproteins, and cross-reactivity was demonstrated by immunologic tests. The data suggest that the chief difference between gp70 and gp45 lies in the carbohydrate content.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Virus Rauscher
/
Proteínas Virales
/
Glicoproteínas
Límite:
Animals
Idioma:
En
Revista:
Biochemistry
Año:
1977
Tipo del documento:
Article
Pais de publicación:
Estados Unidos