Subcellular distribution of aldehyde dehydrogenase activities in human liver.
Alcohol
; 2(1): 107-10, 1985.
Article
en En
| MEDLINE
| ID: mdl-4015824
The subcellular distributions of aldehyde dehydrogenase activities towards acetaldehyde have been determined in wedge-biopsy samples of human liver. A form with Km values of less than 1 microM and 285 microM towards acetaldehyde and NAD+ respectively was present in the mitochondrial fraction. This enzyme had no detectable activity towards N-tele-methylimidazole acetaldehyde, the aldehyde derived from the oxidation of N-tele-methylhistamine. The activity in the cytosol was more sensitive to inhibition by disulfiram and had Km values of 270 microM and 25 microM for acetaldehyde and NAD+, respectively. It was active towards N-tele-methylimidazole acetaldehyde with a Km value of 2.5 microM and a maximum velocity that was 40% of that determined with acetaldehyde. Both these cytosolic activities had alkaline pH optima.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Aldehído Deshidrogenasa
/
Hígado
Límite:
Humans
Idioma:
En
Revista:
Alcohol
Asunto de la revista:
TRANSTORNOS RELACIONADOS COM SUBSTANCIAS
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos