Assay of beta-glucuronidase in bile following ion-pair extraction of pigments and bile acids.
Anal Biochem
; 147(2): 511-6, 1985 Jun.
Article
en En
| MEDLINE
| ID: mdl-4014691
A method for improving the assay of beta-glucuronidase in hepatic and gallbladder bile is described. The method uses ion-pair extraction with N,N,N-triheptyl-1-heptanaminium bromide to remove pigments and bile acids. Conjugated bilirubin, unconjugated bilirubin, and taurine and glycine conjugates of deoxycholic and chenodeoxycholic acids are extracted efficiently from bile by the procedure. The sensitivity of the spectrophotometric assay of beta-glucuronidase in bile using phenolphthalein glucuronide is increased significantly.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bilis
/
Pigmentos Biliares
/
Ácidos y Sales Biliares
/
Glucuronidasa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos