Antibodies to native DNA: detection by immunoperoxidase assay and determination of their immunoglobulin classes.
Clin Chem
; 25(3): 366-70, 1979 Mar.
Article
en En
| MEDLINE
| ID: mdl-400438
Antinuclear antibodies are almost always found in sera of patients with systemic lupus erythematosus. To differentiate antinuclear antibodies from antibodies to DNA in the recently described Crithidia luciliae assay, we developed an immunoperoxidase technique for detecting antibodies to native, double-stranded DNA and compared results by it with those by the Farr assay. Smears of cultured Crithidia luciliae were incubated with human sera, peroxidase-labeled anti-human IgG serum, and diaminobenzidine. The peroxidase stain was examined by conventional light microscopy, which facilitated differentiation between the kinetoplast and the nuclear staining. The Crithidia assay appeared to be specific for double-stranded DNA antibodies, seemed to be more sensitive than the Farr assay, and allowed us to determine the immunoglobulin classes of antibodies to native DNA. Some patients with systemic lupus erythematosus had only IgM or IgA antibodies to DNA.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
ADN
/
Inmunoglobulinas
/
Lupus Eritematoso Sistémico
/
Anticuerpos
Tipo de estudio:
Diagnostic_studies
Límite:
Humans
Idioma:
En
Revista:
Clin Chem
Asunto de la revista:
QUIMICA CLINICA
Año:
1979
Tipo del documento:
Article
Pais de publicación:
Reino Unido