Enhancement of the horseradish peroxidase-catalyzed chemiluminescent oxidation of cyclic diacyl hydrazides by 6-hydroxybenzothiazoles.
Anal Biochem
; 145(1): 96-100, 1985 Feb 15.
Article
en En
| MEDLINE
| ID: mdl-4003766
6-Hydroxybenzothiazole, 2-cyano-6-hydroxybenzothiazole, and 2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid (dehydroluciferin) dramatically enhance light emission from the horseradish peroxidase conjugate catalyzed oxidation of luminol, isoluminol, N-(6-aminobutyl)-N-ethyl isoluminol, and 7-dimethylaminonaphthalene-1,2-dicarboxylic acid hydrazide by either peroxide or perborate. Light emission is enhanced by up to 1000-fold, which is an improvement over the enhancement previously observed using firefly luciferin (4,5-dihydro-2-(6-hydroxy-2-benzothiazolyl)thiazole-4-carboxylic acid). Enhancement is influenced by enhancer concentration and pH. Spectral scans of light emitted in enhanced and unenhanced reactions are similar, suggesting that aminophthalate products, and not the enhancers, are the emitters.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Peroxidasas
/
Tiazoles
/
Peroxidasa de Rábano Silvestre
/
Hidrazinas
/
Indicadores y Reactivos
Idioma:
En
Revista:
Anal Biochem
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos