Explant culture of human fetal small intestine.
Gastroenterology
; 88(3): 691-700, 1985 Mar.
Article
en En
| MEDLINE
| ID: mdl-3967805
Human fetal intestine (10-14 wk gestation) has been cultured as explants in a serum-free Leibovitz L-15 medium for periods up to 9 days. As determined by light microscopy, the overall architecture of the intestinal explant was maintained throughout the culture period. At the ultrastructural level the villus absorptive cells remained tall with well-defined brush border, apical tubular system, and supranuclear and infranuclear accumulations of glycogen. All other epithelial cell types were also preserved. The incorporation of [3H]thymidine and [3H]leucine continued during the culture period, reflecting a sustained synthesis of deoxyribonucleic acid and proteins. The hydrolytic activities of the brush border membrane were established based on data obtained throughout the course of the culture of a large number of intestinal specimens. Sucrase, maltase, glucoamylase, trehalase, lactase, alkaline phosphatase, and gamma-glutamyl transpeptidase activities increased during the 9 days of culture even though different patterns were recorded. These observations clearly established that human fetal small intestine can be maintained in organ culture for at least 9 days in a serum-free medium.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Intestino Delgado
Límite:
Humans
Idioma:
En
Revista:
Gastroenterology
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos