An enzymic method of preparing plant chromosomes for in situ hybridization.
Stain Technol
; 61(1): 21-5, 1986 Jan.
Article
en En
| MEDLINE
| ID: mdl-3952778
A method of preparing chromosomes from plant root tips for in situ hybridization with tritiated DNA is described. The technique relies on the enzymic hydrolysis of plant cell walls with a pectinase-cellulase mixture. It is shown that, despite the enzymic mixture possessing nuclease activity, there is no detectable degradation of DNA within fixed root tips. To demonstrate the suitability of this method of preparing plant chromosomes for in situ hybridization, a cloned repetitive DNA sequence has been hybridized to Allium sativum chromosomes. Chromosomes prepared using this technique also can be readily C-banded.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Plantas
/
Cromosomas
Idioma:
En
Revista:
Stain Technol
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Estados Unidos