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Adaptation of the HistoEnder, an open-source 3D printer for automated transmission electron microscopy grid staining.
Seifert, Philip.
Afiliación
  • Seifert P; Morphology Core, Schepens Eye Research Institute of Mass Eye and Ear Boston, Boston, MA, USA.
J Histotechnol ; : 1-5, 2024 Sep 23.
Article en En | MEDLINE | ID: mdl-39312273
ABSTRACT
The HistoEnder, an inexpensive open-source 3D printer published as an automated histological slide stainer, has been adapted for conventional biological transmission electron microscopy (TEM) batch grid staining. Details are presented of the 3D printed apparatus, assembly, G-code programming, and operation on the 3D printer to post-section stains up to 20 grids through aqueous uranyl acetate, distilled water rinses, and lead stains. TEM Results are identical to manual staining with the advantages of automation using the low cost HistoEnder, apparatus, and equipment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Histotechnol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Histotechnol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido