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N-acetylcysteine mitigates oxidative damage to the ovary in D-galactose-induced ovarian failure in rabbits.
Xue, Yu; Bian, Huafeng; Bai, Shaocheng; Bao, Zhiyuan; Wang, Lei; Wang, Sen; Zhao, Bohao; Wu, Xinsheng; Chen, Yang.
Afiliación
  • Xue Y; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Bian H; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Bai S; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Bao Z; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Wang L; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Wang S; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Zhao B; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Wu X; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China.
  • Chen Y; College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, Jiangsu, China. yangc@yzu.edu.cn.
Mol Biol Rep ; 51(1): 1008, 2024 Sep 23.
Article en En | MEDLINE | ID: mdl-39312076
ABSTRACT

BACKGROUND:

Oxidative damage to the ovaries is the primary cause of impaired reproductive functions in female animals. This study aimed to investigate the protective role of N-Acetyl-L-cysteine (NAC) in reducing oxidative damage in the ovaries of female rabbits. METHODS AND

RESULTS:

Female rabbit ovaries were treated in vitro with varying concentrations of D-galactose (D-gal) 0, 5, 10, and 15 mg/mL, and it was found that 10 mg/mL D-gal significantly disrupted follicular structures, causing disarray in granulosa cell arrangements and significantly reducing T-SOD and GSH levels (p < 0.01). Consequently, we selected 10 mg/mL D-gal to establish an ovarian failure model. These models were treated with multiple doses of NAC (0, 0.1, 0.3, 0.5 mg/mL). The results revealed that the disruption in granulosa cell arrangement caused by 10 mg/mL D-gal was effectively alleviated by 0.1 mg/mL NAC compared to the D-gal treatment group. Furthermore, 10 mg/mL D-gal significantly (p < 0.01) reduced GSH, T-SOD, and catalase (CAT) levels in the ovaries. However, 0.1 mg/mL NAC effectively (p < 0.01) suppressed these adverse effects. Moreover, the current results showed that 10 mg/mL D-gal alone significantly (p < 0.01) downregulated the expression of Nrf2, GPX, PRDX4, GSR, SOD1, and TAF4B, whereas 0.1 mg/mL NAC counteracted these suppressive effects (p < 0.01).

CONCLUSIONS:

It could be concluded that NAC may delay ovarian failure by reducing D-gal-induced ovarian oxidative damage in female rabbit, suggested NAC could be a promising therapeutic agent for protecting against ovarian failure and potentially delaying ovarian failure in female rabbits.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Acetilcisteína / Estrés Oxidativo / Galactosa Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ovario / Acetilcisteína / Estrés Oxidativo / Galactosa Límite: Animals Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos