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Generation and characterization of giant panda induced pluripotent stem cells.
Liu, Yuliang; Zhang, Shihao; Zou, Gaoyang; An, Junhui; Li, Yuan; Lin, Danni; Wang, Donghui; Li, Yan; Chen, Jiasong; Feng, Tongying; Li, Hongyan; Chen, Yijiao; Zhang, Mingyue; Kumar, Manish; Wang, Luqin; Hou, Rong; Liu, Jing.
Afiliación
  • Liu Y; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Zhang S; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
  • Zou G; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
  • An J; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li Y; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
  • Lin D; Guangzhou Regenerative Medicine and Health Guangdong Laboratory at GIBH, Guangzhou 510530, China.
  • Wang D; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Li Y; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
  • Chen J; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Feng T; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
  • Li H; CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangdong-Hong Kong Joint Laboratory for Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China.
  • Chen Y; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Zhang M; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
  • Kumar M; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Wang L; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
  • Hou R; Chengdu Research Base of Giant Panda Breeding, Sichuan Province, Chengdu 610081, China.
  • Liu J; Sichuan Key Laboratory of Conservation Biology for Endangered Wildlife, Sichuan Province, Chengdu 610081, China.
Sci Adv ; 10(38): eadn7724, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39303041
ABSTRACT
The giant panda (Ailuropoda melanoleuca) stands as a flagship and umbrella species, symbolizing global biodiversity. While traditional assisted reproductive technology faces constraints in safeguarding the genetic diversity of giant pandas, induced pluripotent stem cells (iPSCs) known for their capacity to differentiate into diverse cells types, including germ cells, present a transformative potential for conservation of endangered animals. In this study, primary fibroblast cells were isolated from the giant panda, and giant panda iPSCs (GPiPSCs) were generated using a non-integrating episomal vector reprogramming method. Characterization of these GPiPSCs revealed their state of primed pluripotency and demonstrated their potential for differentiation. Furthermore, we innovatively formulated a species-specific chemically defined FACL medium and unraveled the intricate signaling pathway networks responsible for maintaining the pluripotency and fostering cell proliferation of GPiPSCs. This study provides key insights into rare species iPSCs, offering materials for panda characteristics research and laying the groundwork for in vitro giant panda gamete generation, potentially aiding endangered species conservation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ursidae / Diferenciación Celular / Células Madre Pluripotentes Inducidas Límite: Animals Idioma: En Revista: Sci Adv Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ursidae / Diferenciación Celular / Células Madre Pluripotentes Inducidas Límite: Animals Idioma: En Revista: Sci Adv Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos