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Is the Transcription Factor NANOG Involved in Placental Aging?
Farladansky-Gershnabel, Sivan; Silber, Michal; Biron-Shental, Tal; Kovo, Michal; Kidron, Debora; Weisz, Avivit; Zitman-Gal, Tali.
Afiliación
  • Farladansky-Gershnabel S; Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
  • Silber M; Faculty of Medicine & Health Sciences, Tel Aviv University, Tel Aviv, Israel.
  • Biron-Shental T; Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
  • Kovo M; Faculty of Medicine & Health Sciences, Tel Aviv University, Tel Aviv, Israel.
  • Kidron D; Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
  • Weisz A; Faculty of Medicine & Health Sciences, Tel Aviv University, Tel Aviv, Israel.
  • Zitman-Gal T; Department of Obstetrics and Gynecology, Meir Medical Center, Kfar Saba, Israel.
Am J Reprod Immunol ; 92(3): e13927, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39302196
ABSTRACT

PROBLEM:

Accelerated placental aging is linked to abnormal fetal growth, preeclampsia (PE), and preterm birth (PTB). NANOG, a transcription factor, is known for its role in cellular reprogramming, self-renewal, and clonogenic growth. Its expression is regulated by Kruppel-like factor 4 (KLF4), which functions as both a transcriptional activator and repressor. This study evaluated the KLF4-NANOG pathway in placental samples from normal pregnancies (NP) as well as those with PE, fetal growth restriction (FGR), and PTB. METHOD OF STUDY Placental samples from NP pregnancies and those with PE, FGR, and PTB were analyzed for NANOG and KLF4 expression using western blotting and immunohistochemistry.

RESULTS:

NANOG protein expression was significantly increased in placentas from PE, FGR, and PTB compared to NP (fold changes vs. NP PE 2.48 ± 0.3, p = 0.002; FGR 1.64 ± 0.16, p = 0.03; PTB 6.03 ± 3.35, p = 0.01). Similarly, KLF4 protein expression was elevated in PE, FGR, and PTB placentas compared to NP (fold changes vs. NP PE 5.78 ± 0.73, p = 0.001; FGR 2.61 ± 0.43, p = 0.02; PTB 11.42 ± 2.76, p = 0.0006). Immunohistochemistry revealed strong NANOG staining in the syncytiotrophoblast tissue of PE, FGR, and PTB samples, especially in extravillous trophoblasts, compared to NP placentas.

CONCLUSIONS:

The elevated expression of NANOG and KLF4 in abnormal placental tissues suggests their potential role as markers of enhanced placental aging and dysfunction. These findings underscore the importance of the KLF4-NANOG pathway in the pathology of PE, FGR, and PTB, providing a basis for future research into therapeutic targets for these conditions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Factores de Transcripción de Tipo Kruppel / Retardo del Crecimiento Fetal / Proteína Homeótica Nanog / Factor 4 Similar a Kruppel Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Dinamarca
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Placenta / Preeclampsia / Factores de Transcripción de Tipo Kruppel / Retardo del Crecimiento Fetal / Proteína Homeótica Nanog / Factor 4 Similar a Kruppel Límite: Adult / Female / Humans / Pregnancy Idioma: En Revista: Am J Reprod Immunol Año: 2024 Tipo del documento: Article País de afiliación: Israel Pais de publicación: Dinamarca