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Tissue-engineered oral epithelium for dental material testing: towards in vitro biomimetic models.
Machla, Foteini; Monou, Paraskevi Kyriaki; Bekiari, Chrysanthi; Andreadis, Dimitrios; Kofidou, Evangelia; Panteris, Emmanouel; Katsamenis, Orestis L; Kokoti, Maria; Koidis, Petros; About, Imad; Fatouros, Dimitrios; Bakopoulou, Athina.
Afiliación
  • Machla F; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Dentistry, Thessaloniki, Central Macedonia, Greece; fcmachla@dent.auth.gr.
  • Monou PK; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Pharmacy, Thessaloniki, Central Macedonia, Greece.
  • Bekiari C; Center for Interdisciplinary Research and Innovation, Thessaloniki, Greece; paraskemd@pharm.auth.gr.
  • Andreadis D; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Veterinary Medicine, Thessaloniki, Central Macedonia, Greece; chmpekia@vet.auth.gr.
  • Kofidou E; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Dentistry, Thessaloniki, Central Macedonia, Greece; dandrea@dent.auth.gr.
  • Panteris E; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Veterinary Medicine, Thessaloniki, Central Macedonia, Greece; evikofidou@gmail.com.
  • Katsamenis OL; Aristotle University of Thessaloniki Faculty of Sciences, School of Biology, Thessaloniki, Central Macedonia, Greece; epanter@bio.auth.gr.
  • Kokoti M; University of Southampton Faculty of Engineering and Physical Sciences, Biomedical Imaging and X-ray Histology, Southampton, United Kingdom of Great Britain and Northern Ireland.
  • Koidis P; University of Southampton Institute for Life Sciences, Southampton, United Kingdom of Great Britain and Northern Ireland; O.Katsamenis@soton.ac.uk.
  • About I; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Dentistry, Thessaloniki, Central Macedonia, Greece; mkokoti@dent.auth.gr.
  • Fatouros D; Aristotle University of Thessaloniki Faculty of Health Sciences, School of Dentistry, Thessaloniki, Central Macedonia, Greece; pkoidis@dent.auth.gr.
  • Bakopoulou A; Aix-Marseille Université, Marseille, Provence-Alpes-Côte d'Azu, France; imad.about@univ-amu.fr.
Tissue Eng Part C Methods ; 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39302070
ABSTRACT
Tissue-engineered oral epithelium (ΤΕΟΕ) was developed after comparing various culture conditions, including submerged- and air-liquid interface (ALI) human cell expansion options. Barrier formation was evaluated via transepithelial electrical resistance (TEER) and calcein permeation via spectrofluorometry. TEOE was further assessed for long-term viability via live/dead staining and development of intercellular connections via transmission electron microscopy (TEM). Tissue architecture was evaluated via histochemistry and the expression of pancytokeratin (pCK) via immunohistochemistry (IHC). The effect of two commonly used dental resinous monomers on TEOE was evaluated for alterations in cell viability and barrier permeability. ALI/keratinocyte growth factor-supplemented (ALI-KGS) culture conditions led to the formation of an 8-20-layer thick, intercellularly-connected epithelial barrier. TEER values of ALI-KGS-developed TEOE decreased compared to all other tested conditions, and the established epithelium intensively expressed pCK. Exposure to dental monomers affected the integrity and architecture of TEOE, and induced cellular vacuolation, implicating hydropic degeneration. Despite structural modifications, the permeability of TEOE was not substantially affected after exposure to the monomers. In conclusion, the biological properties of the TEOE mimicking the physiological functional conditions and its value as biocompatibility assessment tool for dental materials were characterized.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Tissue Eng Part C Methods Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Tissue Eng Part C Methods Asunto de la revista: BIOTECNOLOGIA / HISTOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos