ARID1A overexpression inhibits colorectal cancer cell migration through the regulation of epithelialmesenchymal transition.
Mol Med Rep
; 30(5)2024 Nov.
Article
en En
| MEDLINE
| ID: mdl-39301629
ABSTRACT
The advancement of tumor cell metastasis is significantly influenced by epithelialtomesenchymal transition (EMT), and metastasis is a prominent contributor to the mortality of patients diagnosed with colorectal cancer (CRC). ATrich interactive domaincontaining protein 1A (ARID1A), which acts as a tumor suppressor, frequently exhibits a lossoffunction mutation in metastatic CRC tissues. However, the underlying molecular mechanisms of ARID1A relating to EMT remain poorly understood. The present study aimed to clarify the association between ARID1A and EMT regulation in human CRC cells. The investigation into the loss of ARID1A expression in tissues from patients with CRC was performed using immunohistochemistry. Furthermore, ARID1Aoverexpressing SW48 cells were established using lentiviruses carrying human fulllength ARID1A. The results revealed that overexpression of ARID1A induced cellular morphological changes by promoting the tight junction molecule zonula occludens 1 (ZO1) and the adherens junction molecule Ecadherin, whereas it decreased the intermediate filament protein vimentin. The results of reverse transcriptionquantitative PCR also confirmed that ARID1A overexpression upregulated the mRNA expression levels of TJP1/ZO1 and CDH1/Ecadherin, and downregulated VIM/vimentin and zinc finger Ebox binding homeobox 1 expression, which are considered epithelial and mesenchymal markers, respectively. In addition, the overexpression of ARID1A in CRC cells resulted in a suppression of cell motility and migratory capabilities. The present study also demonstrated that the tumor suppressor ARID1A was commonly absent in CRC tissues. Notably, ARID1A overexpression could reverse the EMTlike phenotype and inhibit cell migration through alterations in EMTrelated markers, leading to the inhibition of malignant progression. In conclusion, ARID1A may serve as a biomarker and therapeutic target in the clinical management of metastatic CRC.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
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Neoplasias Colorrectales
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Regulación Neoplásica de la Expresión Génica
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Movimiento Celular
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Proteínas de Unión al ADN
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Transición Epitelial-Mesenquimal
Límite:
Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Mol Med Rep
Año:
2024
Tipo del documento:
Article
País de afiliación:
Tailandia
Pais de publicación:
Grecia