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Microdialysis-integrated HPLC system with dual-electrode detection using track-etched membrane electrodes for in vivo monitoring of dopamine dynamics.
Ogawa, Yukuto; Tsugita, Sohei; Torii, Yuka; Iwamoto, Hiten; Sato, Tsukasa; Kasahara, Jiro; Takeuchi, Masaki; Kuwabara, Tomohiko; Iiyama, Masamitsu; Takayanagi, Toshio; Mizuguchi, Hitoshi.
Afiliación
  • Ogawa Y; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan.
  • Tsugita S; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan.
  • Torii Y; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan.
  • Iwamoto H; Faculty of Pharmaceutical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima 770-8505, Japan.
  • Sato T; Faculty of Pharmaceutical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima 770-8505, Japan.
  • Kasahara J; Graduate School of Biomedical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima 770-8505, Japan.
  • Takeuchi M; Graduate School of Biomedical Sciences, Tokushima University, 1-78-1 Shomachi, Tokushima 770-8505, Japan.
  • Kuwabara T; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan.
  • Iiyama M; Nomura Micro Science Co., Ltd., 2-9-10 Okada, Atsugi, Kanagawa 243-0021, Japan.
  • Takayanagi T; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan.
  • Mizuguchi H; Department of Applied Chemistry, Graduate School of Science and Technology, Tokushima University, Tokushima 770-8506, Japan. Electronic address: mizu@tokushima-u.ac.jp.
J Chromatogr B Analyt Technol Biomed Life Sci ; 1247: 124318, 2024 Sep 14.
Article en En | MEDLINE | ID: mdl-39299150
ABSTRACT
A capillary high-performance liquid chromatography (HPLC) system equipped with a dual-electrode detector utilizing track-etched membrane electrodes (TEMEs) was combined with a microdialysis sampling setup. The electrochemical detector benefits from the high electrolysis efficiency of TEMEs, allowing for calibration-free coulometric detection and simplifying data analysis to determine the dopamine recovery through a dialysis probe. Additionally, this system was used for in vivo monitoring of dopamine in the right striatum of a mouse brain. Temporal changes in dopamine levels, including an exponential decay immediately after the dialysis probe insertion and an excess release of dopamine induced by a high concentration of potassium ions, confirmed the system's proper operation. Furthermore, subsequent measurements following the intraperitoneal injection of mirtazapine showed no increase in dopamine levels in the right dorsal striatum. The dual-electrode system displayed characteristic dopamine detection behavior, with anodic and cathodic peak pairs indicative of reversible electrochemical reactions. This capability facilitated the identification of the dopamine peak within the complex chromatogram of the mouse brain dialysate. The consistency between dopamine collection efficiency from standard solutions and dialysate indicated the absence of interfering electroactive substances overlapping with the dopamine peak in the chromatogram. This integrated analysis system successfully tracked temporal fluctuations in dopamine concentration within the mouse brain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chromatogr B Analyt Technol Biomed Life Sci Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Países Bajos