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Novel antibody- and aptamer-based approaches for sensitive detection of mycotoxin fusaric acid in cereal.
Wu, Shih-Wei; Hsieh, Chia-Yu; Liu, Biing-Hui; Lin, Xin-Jie; Yu, Feng-Yih.
Afiliación
  • Wu SW; Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No.1, Sec. 1, Jen Ai Rd, Taipei 100, Taiwan.
  • Hsieh CY; Department of Biomedical Sciences, Chung Shan Medical University, No.110, Sec. 1, Chien Kuo N. Rd, Taichung 402, Taiwan.
  • Liu BH; Graduate Institute of Toxicology, College of Medicine, National Taiwan University, No.1, Sec. 1, Jen Ai Rd, Taipei 100, Taiwan.
  • Lin XJ; Department of Biomedical Sciences, Chung Shan Medical University, No.110, Sec. 1, Chien Kuo N. Rd, Taichung 402, Taiwan.
  • Yu FY; Department of Biomedical Sciences, Chung Shan Medical University, No.110, Sec. 1, Chien Kuo N. Rd, Taichung 402, Taiwan; Department of Medical Research, Chung Shan Medical University Hospital, No.110, Sec. 1, Chien Kuo N. Rd, Taichung 402, Taiwan. Electronic address: fengyu@csmu.edu.tw.
Food Chem ; 463(Pt 2): 141245, 2024 Sep 11.
Article en En | MEDLINE | ID: mdl-39298849
ABSTRACT
This study presents the first successful generation of polyclonal antibodies (pAbs) and oligonucleotide aptamers specifically targeting fusaric acid (FA). Utilizing these pAbs and aptamers, three highly sensitive and specific assays were developed for the detection of FA in cereals with limits of detection (LOD) ranging from 5 to 50 ng/g an antibody-based enzyme-linked immunosorbent assay (ELISA), an aptamer-based enzyme-linked aptamer-sorbent assay (ELASA), and a hybrid enzyme-linked aptamer-antibody sandwich assay (ELAAA). The recovery rates of FA in spiked cereal samples ranged from 87 % to 112 % across all assays. Analysis of 15 cereal feed samples revealed FA contamination levels of 459 to 1743 ng/g (ELISA), 427 to 1960 ng/g (ELASA), and 381 to 1987 ng/g (ELAAA). These results were further validated by HPLC analysis, confirming high consistency within developed assays. Overall, the ELISA, ELASA, and ELAAA are promising tools for the rapid detection of FA, significantly contributing to food safety monitoring.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Food Chem Año: 2024 Tipo del documento: Article País de afiliación: Taiwán Pais de publicación: Reino Unido