Your browser doesn't support javascript.
loading
Atherosclerosis on a Chip: A 3-Dimensional Microfluidic Model of Early Arterial Events in Human Plaques.
Maringanti, Ranganath; van Dijk, Christian G M; Meijer, Elana M; Brandt, Maarten M; Li, Mingzi; Tiggeloven, Vera P C; Krebber, Merle M; Chrifi, Ihsan; Duncker, Dirk J; Verhaar, Marianne C; Cheng, Caroline.
Afiliación
  • Maringanti R; Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, the Netherlands (R.M., C.G.M.v.D., E.M.M., M.M.K., I.C., M.C.V., C.C.).
  • van Dijk CGM; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
  • Meijer EM; Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, the Netherlands (R.M., C.G.M.v.D., E.M.M., M.M.K., I.C., M.C.V., C.C.).
  • Brandt MM; Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, the Netherlands (R.M., C.G.M.v.D., E.M.M., M.M.K., I.C., M.C.V., C.C.).
  • Li M; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
  • Tiggeloven VPC; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
  • Krebber MM; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
  • Chrifi I; Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, the Netherlands (R.M., C.G.M.v.D., E.M.M., M.M.K., I.C., M.C.V., C.C.).
  • Duncker DJ; Department of Nephrology and Hypertension, Regenerative Medicine Center Utrecht, University Medical Center Utrecht, Utrecht University, the Netherlands (R.M., C.G.M.v.D., E.M.M., M.M.K., I.C., M.C.V., C.C.).
  • Verhaar MC; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
  • Cheng C; Experimental Cardiology, Department of Cardiology, Thoraxcenter, Erasmus University Medical Center, Rotterdam, the Netherlands (R.M., M.M.B., M.L., V.P.C.T., I.C., D.J.D., C.C.).
Arterioscler Thromb Vasc Biol ; 2024 Sep 19.
Article en En | MEDLINE | ID: mdl-39297206
ABSTRACT

BACKGROUND:

Realistic reconstruction of the in vivo human atherosclerotic environment requires the coculture of different cell types arranged in atherosclerotic vessel-like structures with exposure to flow and circulating cells, presenting challenges for disease modeling. This study aimed to develop a 3-dimensional tubular microfluidic model with quadruple coculture of human aortic smooth muscle cells, human umbilical cord vein endothelial cells, and foam cells to recreate a complex human atherosclerotic vessel in vitro to study the effects of flow and circulating immune cells.

METHODS:

We developed a coculture protocol utilizing BFP (blue fluorescent protein)-labeled human aortic smooth muscle cells, GFP (green fluorescent protein)-labeled human umbilical cord vein endothelial cells, and THP-1 macrophage-derived, Dil-labeled oxidized LDL (low-density lipoprotein) foam cells within a fibrinogen/collagen I-based 3-dimensional ECM (extracellular matrix). Perfusion experiments were conducted for 24 hours on both atherosclerotic vessels and healthy vessels (BFP-labeled human aortic smooth muscle cells and GFP-labeled human umbilical cord vein endothelial cells without foam cells). Additionally, perfusion with circulating THP-1 monocytes was performed to observe cell extravasation and recruitment.

RESULTS:

The resulting vessels displayed early lesion morphology, with a layered composition including an endothelium and media, and foam cells accumulating in the subendothelial space. The layered wall composition of both atherosclerotic and healthy vessels remained stable under perfusion. Circulating THP-1 monocytes demonstrated cell extravasation into the atherosclerotic vessel wall and recruitment to the foam cell core. The qPCR analysis indicated increased expression of atherosclerosis markers in the atherosclerotic vessels and adaptation of vascular smooth muscle cell migration in response to flow and the plaque microenvironment, compared with control vessels.

CONCLUSIONS:

The human 3-dimensional atherosclerosis model demonstrated stability under perfusion and allowed for the observation of immune cell behavior, providing a valuable tool for the atherosclerosis research field.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Arterioscler Thromb Vasc Biol Asunto de la revista: ANGIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Arterioscler Thromb Vasc Biol Asunto de la revista: ANGIOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos