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Synergistic Assembly of 1DZnO and Anti-CYFRA 21-1: A Physicochemical Approach to Optical Biosensing.
Salinas, Rafael A; Martínez Tolibia, Shirlley E; Zayas-Bazán, Patricia G; Rodil, Sandra E; Mathew, Mathew T; Navarrete, Andrés; Santana, Guillermo; Dutt, Ateet.
Afiliación
  • Salinas RA; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  • Martínez Tolibia SE; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  • Zayas-Bazán PG; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  • Rodil SE; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  • Mathew MT; Department of Biomedical Science, UIC College of Medicine, Rockford, IL 61107, USA.
  • Navarrete A; Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México, Ciudad Universitaria C.P. 04510, Coyoacán, Mexico City, México.
  • Santana G; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
  • Dutt A; Departamento de Materiales de Baja Dimensionalidad, Instituto de Investigaciones en Materiales Universidad Nacional Autónoma de México, C.P. 04510, Mexico City, México.
BME Front ; 5: 0064, 2024.
Article en En | MEDLINE | ID: mdl-39297176
ABSTRACT

Objective:

We conducted a comprehensive physicochemical analysis of one-dimensional ZnO nanowires (1DZnO), incorporating anti-CYFRA 21-1 immobilization to promote fast optical biomarker detection up to 10 ng ml-1. Impact Statement This study highlights the effectiveness of proof-of-concept 1DZnO nanoplatforms for rapid cancer biomarker detection by examining the nanoscale integration of 1DZnO with these bioreceptors to deliver reliable photoluminescent output signals.

Introduction:

The urgent need for swift and accurate prognoses in healthcare settings drives the rise of sensitive biosensing nanoplatforms for cancer detection, which has benefited from biomarker identification. CYFRA 21-1 is a reliable target for the early prediction of cancer formation that can be perceptible in blood, saliva, and serum. However, 1DZnO nanostructures have been barely applied for CYFRA 21-1 detection.

Methods:

We assessed the nanoscale interaction between 1DZnO and anti-CYFRA 21-1 antibodies to develop rapid CYFRA 21-1 detection in two distinct matrices PhosphateBuffered Saline (PBS) buffer and artificial saliva. The chemical modifications were tracked utilizing Fourier transform infrared spectroscopy, while transmission electron microscopy and energy dispersive spectroscopy confirmed antigen-antibody interplay over nanostructures.

Results:

Our results show high antibody immobilization efficiencies, affirming the effectiveness of 1DZnO nanoplatforms for rapid CYFRA 21-1 testing within a 5-min detection window in both PBS and artificial saliva. Photoluminescence measurements also revealed distinct optical responses across biomarker concentrations ranging from 10 to 1,000 ng ml-1.

Conclusion:

Discernible PL signal responses obtained after 5 min affirm the potential of 1DZnO nanoplatforms for further advancement in optical biomarker detection for application in early cancer prognosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BME Front Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: BME Front Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos