UDP-glucuronosyltransferase: fluorometric determination and microcomputer method for bisubstrate kinetics analysis.
Anal Biochem
; 148(1): 37-43, 1985 Jul.
Article
en En
| MEDLINE
| ID: mdl-3929644
A method for analysing UDP-glucuronosyltransferase activity in rainbow trout hepatic microsomes is described, using 1-naphthol as a substrate and fluorometric determination of glucuronide. Kinetic constants are computed with a classical plot in a weighted regression. The computer uses the least-squares method for each value of a variable which is set and incremented. To get confidence intervals, the computer generates random values around experimental data (in a confidence interval they determine), and then computes again. With each simulation, a weighted regression with classical secondary plots gives simulated kinetic parameters. From each population of simulated values, an interval containing a given percentage of the population is determined.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Glucuronosiltransferasa
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
1985
Tipo del documento:
Article
Pais de publicación:
Estados Unidos