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Evaluation of the GenoType NTM-DR line probe assay for nontuberculous mycobacteria using whole genome sequences as reference standard.
Epperson, L Elaine; Davidson, Rebecca M; Kammlade, Sara M; Hasan, Nabeeh A; Nick, Sophie E; Machado, Iara M P; Rodriguez, Valerie H; Appleman, Aaron; Helstrom, Niels K; Strong, Michael.
Afiliación
  • Epperson LE; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA. Electronic address: eppersone@njhealth.org.
  • Davidson RM; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA.
  • Kammlade SM; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA.
  • Hasan NA; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA.
  • Nick SE; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA.
  • Machado IMP; Advanced Diagnostic Laboratories, National Jewish Health, Denver, CO, USA.
  • Rodriguez VH; Advanced Diagnostic Laboratories, National Jewish Health, Denver, CO, USA.
  • Appleman A; Advanced Diagnostic Laboratories, National Jewish Health, Denver, CO, USA.
  • Helstrom NK; Advanced Diagnostic Laboratories, National Jewish Health, Denver, CO, USA.
  • Strong M; Center for Genes, Environment and Health, National Jewish Health, 1600 Jackson Street, Denver, CO, USA.
Diagn Microbiol Infect Dis ; 110(4): 116526, 2024 Sep 11.
Article en En | MEDLINE | ID: mdl-39293318
ABSTRACT
Pulmonary nontuberculous mycobacteria (NTM) disease is an emerging public health challenge that is especially problematic in people with cystic fibrosis (CF). Effective treatment depends on accurate species and subspecies identification and antimicrobial susceptibility status. We evaluated the GenoType NTM-DR VER 1.0 assay using biobanked NTM isolates with whole genome sequence (WGS) data and control isolates (total n=285). Species and subspecies detection sensitivity and specificity were 100 % for all species and subspecies except for two subspecies of M. intracellulare, that demonstrated a small degree of discrepant identification between M. intracellulare subspecies intracellulare and subspecies chimaera. All antimicrobial resistance markers were identified with 100 % sensitivity and specificity. We conclude that the GenoType NTM-DR assay offers a rapid and accurate option for identifying the most frequently encountered pathogenic NTM taxa and drug resistance markers. SUPPORT Colorado CF Research Development Program and Colorado CF National Resource Centers funded by the Cystic Fibrosis Foundation, NJH Advanced Diagnostics Laboratories, Colorado Advanced Industries Accelerator Grant.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Diagn Microbiol Infect Dis Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos