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Role of Moringa oleifera irrigation solution on the cell metabolism change of Streptococcus mutans.
Soraya, Cut; Batubara, Fitri Yunita; Nasroen, Saskia L; Jakfar, Subhaini; Gani, Basri A.
Afiliación
  • Soraya C; Department of Dentistry Conservative, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia.
  • Batubara FY; Department of Conservative Dentistry, Dentistry Faculty, University of Sumatera Utara, Medan, Sumatera Utara, Indonesia.
  • Nasroen SL; Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Jenderal Achmad Yani, Cimahi, Bandung, Indonesia.
  • Jakfar S; Department of Dental Material, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia.
  • Gani BA; Department of Oral Biology, Dentistry Faculty, University of Syiah Kuala, Darussalam, Banda Aceh, Aceh, Indonesia.
J Adv Pharm Technol Res ; 15(3): 200-207, 2024.
Article en En | MEDLINE | ID: mdl-39290550
ABSTRACT
The principal etiological agent responsible for dental caries is Streptococcus mutans (S. mutans). The Moringa oleifera (M. oleifera) possesses antioxidant and antibacterial properties that function through the response to oxidative stress, which affects bacterial cell metabolism. This research examined M. oleifera impact on S. mutans growth, toxicity, glucan-binding protein (GBP) expression, and nucleic acid structure. Methods included spectrophotometry for growth analysis, enzyme-linked immunosorbent assay for GBP quantification, the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) MTT assay for cytotoxicity, Fourier transform infrared for nucleic acid changes, and docking simulation for ligand-receptor affinity. Results showed that M. oleifera significantly inhibited S. mutans growth at all concentrations over 24 and 48 h (optical density <0.1), comparable to <300 CFU/mL. At 72 h, 6.25% and 3.125% concentrations were most effective, with chlorhexidine also showing stability at these times. A 3.125% concentration of M. oleifera notably reduced GBP production to below 15% and caused cell toxicity. Furthermore, 25% and 3.125% concentrations significantly altered S. mutans nucleic acids, and M. oleifera showed high binding affinity to the GBP gene receptor. Thus, M. oleifera can inhibit S. mutans growth and GBP production, cause nucleic acid deformation, and strongly bind to the GBP receptor, highlighting its potential in dental caries prevention.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Adv Pharm Technol Res Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Adv Pharm Technol Res Año: 2024 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: India