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miR-30c-5p inhibits esophageal squamous cell carcinoma progression by repressing the PI3K/AKT signaling pathway.
Shi, Haochun; Pan, Binyang; Liang, Jiaqi; Cai, Benjie; Wu, Gujie; Bian, Yunyi; Shan, Guangyao; Ren, Shencheng; Huang, Yiwei; Guo, Weigang.
Afiliación
  • Shi H; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Pan B; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Liang J; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Cai B; Department of Thoracic Surgery and Urology, Shigatse People's Hospital, Shigatse, China.
  • Wu G; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Bian Y; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Shan G; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Ren S; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Huang Y; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
  • Guo W; Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai, China.
Thorac Cancer ; 2024 Sep 17.
Article en En | MEDLINE | ID: mdl-39289835
ABSTRACT

BACKGROUND:

Esophageal squamous cell carcinoma (ESCC) is one of the most common malignant tumors, with high incidence and poor prognosis. Revealing mechanisms of ESCC progression and developing new therapeutic targets remains crucial. The aim of this study was to elucidate the molecular mechanism of miR-30c-5p in regulating the malignant progression of ESCC.

METHODS:

TCGA, GEO, and other datasets were used to analyze the differential expression of miR-30c-5p in ESCC and adjacent tissues, and its impact on prognosis. Then the effects of miR-30c-5p on the proliferation, migration, and invasion of TE-1 and Eca9706 cells were investigated through proliferation experiments, transwell and wounding healing assays. The regulatory mechanism of miR-30c-5p on the PI3K/AKT signaling pathway and its interaction in cancer progression were investigated through Western blots, dual-luciferase reporter assay, and rescue experiments.

RESULTS:

miR-30c-5p was significantly downregulated in ESCC tissue and represented a poor prognosis. miR-30c-5p mimic significantly inhibited the proliferation, migration, and invasion ability of ESCC, while miR-30c-5p inhibitor significantly promoted tumor cell progression. Through bioinformatic analysis and experimental results, miR-30c-5p interacted directly with PIK3CA mRNA and inhibited subsequent signaling pathway activation. PIK3CA activator could eliminate the inhibitory effects of miR-30c-5p mimic on the progression of ESCC, while PIK3CA inhibitors could rescue the promoting effect of miR-30c-5p inhibitor group cells.

CONCLUSIONS:

In summary, we found that miR-30c-5p inhibited the proliferation, invasion and migration of ESCC by inhibiting PI3K/AKT signaling pathway for the first time, and this study is expected to provide a novel insight and potential therapeutic target for managing ESCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Thorac Cancer Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Thorac Cancer Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Singapur