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Molecular biomarkers involved in the progression of gallbladder inflammatory lesions to invasive cancer: A proteomic approach.
Rawal, Neetu; Hariprasad, Gururao; Bandyopadhyay, Sabyasachi; Ranjan Dash, Nihar; Kumar, Sunil; Das, Prasenjit; Dey, Sharmistha; Ahmad Khan, Maroof; Ranjan, Amar; Chopra, Anita; Saluja, Sundeep; Hussain, Showket; Rath, G K; Kaur, Tanvir; Tanwar, Pranay.
Afiliación
  • Rawal N; Laboratory Oncology Unit, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
  • Hariprasad G; Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
  • Bandyopadhyay S; Proteomics Laboratory, Centralized Core Research Facility, All India Institute of Medical Sciences, New Delhi, India.
  • Ranjan Dash N; Department of Gastrointestinal Surgery, All India Institute of Medical Sciences, New Delhi, India.
  • Kumar S; Department of Surgical Oncology, All India Institute of Medical Sciences, New Delhi, India.
  • Das P; Department of Pathology, All India Institute of Medical Sciences, New Delhi, India.
  • Dey S; Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
  • Ahmad Khan M; Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India.
  • Ranjan A; Laboratory Oncology Unit, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
  • Chopra A; Laboratory Oncology Unit, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
  • Saluja S; Department of Gastrointestinal Surgery, Govind Ballabh Pant Hospital, New Delhi, India.
  • Hussain S; Division of Molecular Oncology, National Institute of Cancer Prevention and Research, Indian Council of Medical Research, New Delhi, India.
  • Rath GK; Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
  • Kaur T; Division of Non-Communicable Diseases, Indian Council of Medical Research, New Delhi, India.
  • Tanwar P; Laboratory Oncology Unit, Dr. B.R.A. Institute Rotary Cancer Hospital, All India Institute of Medical Sciences, New Delhi, India.
Biomol Biomed ; 2024 Sep 08.
Article en En | MEDLINE | ID: mdl-39284282
ABSTRACT
The progression of gallbladder inflammatory lesions to invasive cancer remains poorly understood, necessitating research on biomarkers involved in this transition. This study aims to identify and validate proteins associated with this progression, offering insights into potential diagnostic biomarkers for gallbladder cancer (GBC). Label-free liquid chromatography assisted tandem mass spectrometry (LC-MS/MS) proteomics was performed on samples from 10 cases each of GBC and inflammatory lesions, with technical duplicates. Validation was conducted through the enzyme-linked immunosorbent assay (ELISA) using 80 samples (40 GBC and 40 inflammatory lesions). Bioinformatics tools analyzed protein-protein interaction (PPI) networks and pathways. Statistical correlations with clinicopathological variables were assessed. Prognostic evaluation utilized Kaplan-Meier survival analysis and Cox regression analyses. mRNA expressions were studied using real time-polymerase chain reaction (RT-PCR). Out of 5,714 proteins analyzed, 621 were differentially expressed. Three upregulated (the S100 calcium-binding protein P [S100P], polymeric immunoglobulin receptor [PIGR], and complement C1q-binding protein [C1QBP]) and two downregulated (transgelin [TAGLN] and calponin 1 [CNN1]) proteins showed significant expression. Pathway analysis implicated involvement of proteoglycans in cancer and glycosaminoglycan metabolism. Significant correlations were observed between protein concentrations and clinicopathological variables. Prognostic factors such as tumor size, lymph node metastasis, and preoperative bilirubin levels were associated with overall survival. Protein-based assays demonstrated higher resolution compared to mRNA analysis, suggesting their utility in GBC risk stratification. S100P, PIGR, C1QBP, TAGLN, and CNN1 emerge as potential protein-based biomarkers involved in the progression from gallbladder inflammatory lesions to invasive cancer. These findings hold promise for improved diagnostic and prognostic strategies in GBC management.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomol Biomed Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Bosnia-Herzegovina
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomol Biomed Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: Bosnia-Herzegovina