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Identification of Retinal Amyloid-Beta in Ex-Vivo Human Glaucoma Eyes Using a Novel Ocular Tracer.
Pilotte, Julie; Khoury, Sami; Tafreshi, Ali; Mandel, Zachary T; Sharma, Svasti V; Vanderklish, Peter; Sarraf, Stella T; Sadun, Alfredo A; Weinreb, Robert N; Huang, Alex S.
Afiliación
  • Pilotte J; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Khoury S; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Tafreshi A; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Mandel ZT; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Sharma SV; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Vanderklish P; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Sarraf ST; Amydis, Inc., 9310 Athena Circle, Suite 100, La Jolla, CA USA.
  • Sadun AA; Doheny Eye Institute, Los Angeles, CA; Department of Ophthalmology, David Geffen School of Medicine at UCLA, Los Angeles, CA.
  • Weinreb RN; Hamilton Glaucoma Center, The Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California, La Jolla, CA, USA.
  • Huang AS; Hamilton Glaucoma Center, The Viterbi Family Department of Ophthalmology, Shiley Eye Institute, University of California, La Jolla, CA, USA.
J Glaucoma ; 2024 Sep 17.
Article en En | MEDLINE | ID: mdl-39283690
ABSTRACT

PURPOSE:

To characterize the presence of amyloid-beta (Aß) in human glaucoma retina and to test identification of retinal Aß using a novel fluorescent Aß-binding small molecule (AMDX-2011).

METHODS:

Post-mortem human eyes with (n=4) and without (n=4) glaucoma were acquired from an eye bank. Retinas were dissected, flat-mounted, and fixed. Using the flat-mounts, immunofluorescence was performed against Aß, AMDX-2011 staining was conducted, and images were acquired using fluorescence microscopy.

RESULTS:

Fluorescence microscopy demonstrated presence of Aß signal that co-localized with AMDX-2011 staining in glaucoma retina. Co-labeled puncta appeared in all quadrants of the retina, including retina temporal to the optic nerve. The puncta were mainly located within the inner layers of the retina. Glaucoma retinas had more co-labeled puncta than control retinas in all locations (P = 0.002-0.02). Co-labeled puncta were also larger in the superior quadrant of glaucoma compared to control retinas (P = 0.02).

CONCLUSIONS:

Aß was detected in human glaucomatous retina, and its distribution was mapped. AMDX-2011 identification of Aß may lead to future diagnostic tests aimed at detecting Aß in glaucoma patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Glaucoma Asunto de la revista: OFTALMOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Glaucoma Asunto de la revista: OFTALMOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos