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Non-invasive, pre-symptomatic detection of potato cyst nematode infection in tomato using chlorophyll fluorescence analysis.
van Himbeeck, Robbert; Binnebösz, Eline Laura; Amora, Deisy; Gottardi, Michele; Willig, Jaap-Jan; Geisen, Stefan; Helder, Johannes.
Afiliación
  • van Himbeeck R; Wageningen University & Research, Laboratory of Nematology, Wageningen, Netherlands; robbert.vanhimbeeck@wur.nl.
  • Binnebösz EL; Wageningen University & Research, Laboratory of Nematology, Wageningen, Netherlands; elinelaura.binnebosz@wur.nl.
  • Amora D; Novonesis A/S, Plant Biosolutions Applied R&D, Højbakkegård Alle, 20 - 2630 - Taastrup, Taastrup, Denmark, 2630; deam@novonesis.com.
  • Gottardi M; Novonesis A/S, Plant Biosolutions Applied R&D, Taastrup, Denmark; mgot@novonesis.com.
  • Willig JJ; Wageningen University & Research, Laboratory of Nematology, Droevendaalsesteeg 1, Wageningen, Netherlands, 6708PB.
  • Geisen S; Wageningen University & Research, Agrosystems Research, Droevendaalsesteeg 1, Wageningen, Gelderland, Netherlands, 6708PB; jaap-jan.willig@wur.nl.
  • Helder J; Wageningen University & Research, Laboratory of Nematology, Wageningen, Netherlands; stefan.geisen@wur.nl.
Phytopathology ; 2024 Sep 16.
Article en En | MEDLINE | ID: mdl-39283194
ABSTRACT
Potato cyst nematodes (PCN) are notorious pathogens in all major potato production areas worldwide. Mainly due to the low mobility of this soil pathogen, PCN infestations are mostly observed as patches ('foci') that only cover a fraction of the acreage. In-field pre-symptomatic localization of this pathogen is valuable as it would allow for the localized application of control measures. Although the mapping of foci is technically feasible, it is unpractical as it would take the analysis of numerous soil samples. We investigated whether chlorophyll fluorescence (Chl-F) could be suitable as a rapid, non-destructive method for early PCN detection. To this end, the impact of four Globodera pallida densities on the Chl-F of tomato was investigated in a phenotyping greenhouse for 26 days. Furthermore, classical plant performance indicators biomass and root surface area were compared with Chl-F. Thermal dissipation ('NPQ_Lss') and actual photosynthetic rate ('QY_Lss') responded at 1 DPI, while QY_Lss was most sensitive to low PCN infection levels. Chl-F parameters responded more readily to PCN infection than biomass and root surface area. The efficiency of photosystem II (QY_max) and the potential activity of photosystem II (Fv/Fo) initially increased at low PCN infection levels, whereas a sharp decrease was observed at higher infestation levels. Hence, our data suggest that low PCN levels promoted plant performance before becoming detrimental at higher levels. While Chl-F allowed for early and sensitive PCN detection, it remains to be investigated whether these signals can be distinguished from those produced by other below-ground stressors in the field.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Phytopathology Asunto de la revista: BOTANICA Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos