Your browser doesn't support javascript.
loading
Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model.
Hu, Jing; Shi, Zheng-Shuai; Liu, Xiang-Zhong; Cai, Han-Tao; Yang, Ao-Fei; Sun, Da-Ming; Xu, Liang-Liang; Yang, Yi; Li, Zhang-Hua.
Afiliación
  • Hu J; Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430015, China.
  • Shi ZS; Huanggang Hospital of TCM Affiliated to Hubei University of Chinese Medicine, Huanggang 438000, China.
  • Liu XZ; Department of Orthopedics, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, China.
  • Cai HT; Hubei University of Chinese Medicine, Wuhan 430061, China.
  • Yang AF; Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, China.
  • Sun DM; Wuhan Sports University, Wuhan 430079, China.
  • Xu LL; Wuhan Sports University, Wuhan 430079, China.
  • Yang Y; Wuhan Sports University, Wuhan 430079, China.
  • Li ZH; Department of Orthopedics, Wuhan Third Hospital, Tongren Hospital of Wuhan University, Wuhan 430060, China.
Stem Cells Int ; 2022: 6865041, 2022.
Article en En | MEDLINE | ID: mdl-39282499
ABSTRACT

Objectives:

Osteoarthritis is the leading disease of joints worldwide. Osteoarthritis may be treated by exosomes derived from Runx2-overexpressed bone marrow mesenchymal stem cells (R-BMSCs-Exos). R-BMSCs-Exos would promote the proliferation, migration, and phenotypic maintenance of articular chondrocytes.

Methods:

BMSCs were transfected with and without Runx2. Exosomes derived from BMSCs and R-BMSCs (BMSCs-Exos and R-BMSCs-Exos) were isolated and identified. Proliferation, migration, and phenotypic maintenance were determined in vitro and compared between groups. The mechanism for activation of Yes-associated protein (YAP) was investigated using small interfering RNA (siRNA). The exosomes' preventive role was determined in vivo using Masson trichrome and immunohistochemical staining.

Results:

R-BMSCs-Exos enhance the proliferation, migration, and phenotypic maintenance of articular chondrocytes based on the YAP being activated. R-BMSCs-Exos prevent knee osteoarthritis as studied in vivo through a rabbit model.

Conclusions:

Findings emphasize the efficacy of R-BMSCs-Exos in preventing osteoarthritis. Potential source of exosomes is sorted out for the advantages and shortcomings. The exosomes are then modified based on the molecular mechanisms to address their limitations. Such exosomes derived from modified cells have the role in future therapeutics.
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Stem Cells Int Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Stem Cells Int Año: 2022 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos