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The therapeutic potential and molecular mechanism of Alpha-pinene, Gamma-terpinene, and P-cymene against melanoma cells.
Acikgul, Funda Cimen; Duran, Nizami; Kutlu, Tuncer; Ay, Emrah; Tek, Erhan; Bayraktar, Suphi.
Afiliación
  • Acikgul FC; Department of Medical Microbiology, Medical Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
  • Duran N; Department of Medical Microbiology, Medical Faculty, Agri Ibrahim Cecen University, Agri 04100, Turkiye.
  • Kutlu T; Department of Medical Microbiology, Medical Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
  • Ay E; Department of Pathology, Veterinary Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
  • Tek E; Department of Medical Microbiology, Medical Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
  • Bayraktar S; Department of Medical Microbiology, Medical Faculty, Mustafa Kemal University, Antakya-Hatay, 31060, Turkey.
Heliyon ; 10(17): e36223, 2024 Sep 15.
Article en En | MEDLINE | ID: mdl-39281661
ABSTRACT
The purpose of this study is to investigate the potential anticarcinogenic effects of three phytochemicals, namely Alpha-pinene (AP), Gamma-terpinene (GT), and P-cymene (PC), on melanoma cells (A2058 cell line). Additionally, the study aims to explore the synergistic activities of these phytochemicals with Dacarbazine, a chemotherapy drug. To understand the molecular mechanism involved in apoptosis induction in the A-2058 cell line, it was used AO/EB staining for apoptosis detection and cell cycle analysis, monitored through flow cytometry. It also determined the mRNA expression levels of different apoptosis-regulatory genes, including p53, Bax, NF-kB, Bcl-2, Bcl-xl, and caspase-3. The antitumor activities of these phytochemicals and their combinations were investigated in a subcutaneous mouse tumor model. The tumor diameter was 21.4 ± 1.1 mm in the Dacarbazine treatment group and 42.4 ± 3.1 mm in the control group. The antitumoral activities of AP and PC in the tumor model were similar to those of Dacarbazine. On the other hand, GT exhibited remarkable antitumoral activity, with a 1.75-fold reduction in tumor diameter compared to the Dacarbazine group. When different combinations of phytochemicals and Dacarbazine were used, the GT plus Dacarbazine treatment group was found to have a 3.5-fold reduction in tumor diameter compared to the Dacarbazine group. The tumor diameters in the Dacarbazine, AP plus GT, GT plus Dacarbazine, and AP plus Dacarbazine treatment groups were 21.4 ± 1.1, 7.6 ± 2.2, 8.6 ± 0.5, and 6.2 ± 1.9 mm, respectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2024 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido