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Heterologous expression of the maize transcription factor ZmbHLH36 enhances abiotic stress tolerance in Arabidopsis.
Dai, Zhenggang; Zhao, Keyong; Zheng, Dengyu; Guo, Siyu; Zou, Huawen; Wu, Zhongyi; Zhang, Chun.
Afiliación
  • Dai Z; College of Agriculture, Yangtze University, Jingzhou, 434025 China.
  • Zhao K; Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097 China.
  • Zheng D; Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097 China.
  • Guo S; Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097 China.
  • Zou H; College of Agriculture, Yangtze University, Jingzhou, 434025 China.
  • Wu Z; Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing Key Laboratory of Agricultural Gene Resources and Biotechnology, Beijing, 100097 China.
  • Zhang C; College of Agriculture, Yangtze University, Jingzhou, 434025 China.
aBIOTECH ; 5(3): 339-350, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39279862
ABSTRACT
Basic helix-loop-helix (bHLH) transcription factors are widely distributed in eukaryotes, and in plants, they regulate many biological processes, such as cell differentiation, development, metabolism, and stress responses. Few studies have focused on the roles of bHLH transcription factors in regulating growth, development, and stress responses in maize (Zea mays), even though such information would greatly benefit maize breeding programs. In this study, we cloned the maize transcription factor gene ZmbHLH36 (Gene ID 100193615, GRMZM2G008691). ZmbHLH36 possesses conserved domains characteristic of the bHLH family. RT-qPCR analysis revealed that ZmbHLH36 was expressed at the highest level in maize roots and exhibited different expression patterns under various abiotic stress conditions. Transgenic Arabidopsis (Arabidopsis thaliana) plants heterologously expressing ZmbHLH36 had significantly longer roots than the corresponding non-transgenic plants under 0.1 and 0.15 mol L-1 NaCl treatment as well as 0.2 mol L-1 mannitol treatment. Phenotypic analysis of soil-grown plants under stress showed that transgenic Arabidopsis plants harboring ZmbHLH36 exhibited significantly enhanced drought tolerance and salt tolerance compared to the corresponding non-transgenic plants. Malondialdehyde contents were lower and peroxidase activity was higher in ZmbHLH36-expressing Arabidopsis plants than in the corresponding non-transgenic plants. ZmbHLH36 localized to the nucleus when expressed in maize protoplasts. This study provides a systematic analysis of the effects of ZmbHLH36 on root growth, development, and stress responses in transgenic Arabidopsis, laying a foundation for further analysis of its roles and molecular mechanisms in maize. Supplementary Information The online version contains supplementary material available at 10.1007/s42994-024-00159-3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ABIOTECH Año: 2024 Tipo del documento: Article Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: ABIOTECH Año: 2024 Tipo del documento: Article Pais de publicación: Singapur