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Relationship between systemic biomarker of lipid peroxidation 4-hydroxynonenal and lipidomic profile of morbidly obese patients undergoing bariatric surgery.
Halasz, Mirna; Luczaj, Wojciech; Jarocka-Karpowicz, Iwona; Stasiewicz, Anna; Soldo, Ana Maria; Soldo, Ivo; Pajtak, Alen; Sencar, Marin; Grgic, Tihomir; Kolak, Toni; Zarkovic, Neven; Skrzydlewska, Elzbieta; Jaganjac, Morana.
Afiliación
  • Halasz M; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia.
  • Luczaj W; Department of Analytical Chemistry, Medical University of Bialystok, 15-222 Bialystok, Poland.
  • Jarocka-Karpowicz I; Department of Analytical Chemistry, Medical University of Bialystok, 15-222 Bialystok, Poland.
  • Stasiewicz A; Department of Analytical Chemistry, Medical University of Bialystok, 15-222 Bialystok, Poland.
  • Soldo AM; Internal Medicine Clinic, University Hospital "Sveti Duh", Zagreb, Croatia.
  • Soldo I; Surgery Clinic, University Hospital "Sveti Duh", Zagreb, Croatia.
  • Pajtak A; Department of Abdominal Surgery, General Hospital Varazdin, Varazdin, Croatia.
  • Sencar M; Department of Abdominal Surgery, General Hospital Varazdin, Varazdin, Croatia.
  • Grgic T; Surgery Clinic, University Hospital "Sveti Duh", Zagreb, Croatia.
  • Kolak T; Department of Abdominal Surgery, University Hospital Dubrava, Zagreb, Croatia.
  • Zarkovic N; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia.
  • Skrzydlewska E; Department of Analytical Chemistry, Medical University of Bialystok, 15-222 Bialystok, Poland. Electronic address: elzbieta.skrzydlewska@umb.edu.pl.
  • Jaganjac M; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia. Electronic address: morana.jaganjac@irb.hr.
Free Radic Biol Med ; 224: 564-573, 2024 Sep 13.
Article en En | MEDLINE | ID: mdl-39278574
ABSTRACT
Obesity is characterized by fat accumulation, impaired metabolism and oxidative stress, frequently associated with lipid peroxidation and generation of bioactive 4-hydroxynonenal (4-HNE). This study aimed to evaluate the impact of bariatric surgery-induced weight loss on lipid peroxidation and associated perturbations in lipid profile. Plasma samples of twenty obese individuals before and 6 months after bariatric surgery were collected in addition to samples of ten healthy controls. HILIC-LC-MS/MS platform was used to characterize phospholipid profile, while lipid peroxidation markers 15-F2t-IsoP, 10-F4t-NeuroP and reactive aldehyde 4-HNE were quantified by RP-LC-MS/MS and GC-MS, respectively. Six months post-surgery lipid peroxidation markers decreased significantly and the BMI of morbidly obese patients decreased by 13 on average. Lipidomics analysis, identified 117 phospholipid species from seven classes, and showed obesity-associated lipidome perturbations, particularly in ether-linked phosphatidylethanolamines (PEo). A total of 45 lipid species were found to be significantly altered with obesity, while 10 lipid species correlated with lipid peroxidation markers. Sample pairwise analyses indicated an interesting link between 4-HNE and the amount of two PEos, PEo (382) and PEo (362). The results indicate that weight loss-induced improvement of redox homeostasis together with changes in lipid metabolites may serve as markers of metabolic improvement. However, further studies are needed to understand the role of obesity-induced oxidative stress on ether lipid biosynthesis and lipidome perturbations, as well as the impact of bariatric surgery on metabolic improvement.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Free Radic Biol Med Asunto de la revista: BIOQUIMICA / MEDICINA Año: 2024 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Estados Unidos