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Endosperm-specific expressed transcription factor protein WRINKLED1-mediated oil accumulative mechanism in woody oil peony Paeonia ostii var. lishizhenii.
Li, Linkun; Zhang, Wei; Xu, Shiming; Li, Yipei; Xiu, Yu; Wang, Huafang.
Afiliación
  • Li L; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. Electronic address: lilinkun1114@caf.ac.cn.
  • Zhang W; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. Electronic address: zhangweipipa@163.com.
  • Xu S; Department of Biochemistry and Molecular Biology, Yanjing Medical College, Capital Medical University, Beijing 101300, China. Electronic address: xushiming@ccmu.edu.cn.
  • Li Y; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: liyipei0111@163.com.
  • Xiu Y; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: yuxiu@bjfu.edu.cn.
  • Wang H; College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China. Electronic address: hfwang@bjfu.edu.cn.
Plant Sci ; 349: 112266, 2024 Sep 13.
Article en En | MEDLINE | ID: mdl-39278569
ABSTRACT
Paeonia ostii var. lishizhenii exhibits superiority of high α-linolenic acid in seed oils, yet, the low yield highlights the importance of enhancing oil accumulation in seeds for edible oil production. The transcription factor protein WRINKLED1 (WRI1) plays crucial roles in modulating oil content in higher plants; however, its functional characterization remains elusive in P. ostii var. lishizhenii. Herein, based on a correlation analysis of transcription factor transcript levels, FA accumulation rates, and interaction assay of FA biosynthesis associated proteins, a WRI1 homologous gene (PoWRI1) that potentially regulated oil content in P. ostii var. lishizhenii seeds was screened. The PoWRI1 exhibited an endosperm-specific and development-depended expression pattern, encoding a nuclear-localized protein with transcriptional activation capability. Notably, overexpressing PoWRI1 upregulated certain key genes relevant to glycolysis, FA biosynthesis and desaturation, and improved seed development, oil body formation and oil accumulation in Arabidopsis seeds, resulting an enhancement of total seed oil weight by 9.47-18.77 %. The defective impacts on seed phenotypes were rescued through ectopic induction of PoWRI1 in wri1 mutants. Our findings highlight the pivotal role of PoWRI1 in controlling oil accumulation in P. ostii var. lishizhenii, offering bioengineering strategies to increase seed oil accumulation and enhance its potential for edible oil production.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Sci Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Plant Sci Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda