Your browser doesn't support javascript.
loading
Alternating magnetic fields (AMF) and linezolid reduce Staphylococcus aureus biofilm in a large animal implant model.
Somawardana, Isuru A; Prasad, Bibin; Kay, Walker; Hunt, Connor; Adams, Jacob; Kawaguchi, Brooke; Smith, Tyler B; Ashton, Nicholas; Sadaphal, Varun; Tepper, John; Monogue, Marguerite; Ramirez, Josue I; Jones, Olivia D; Shelton, John M; Evers, Bret M; Serge, Rousselle; Pybus, Christine; Williams, Dustin; Chopra, Rajiv; Greenberg, David E.
Afiliación
  • Somawardana IA; Texas A&M School of Engineering Medicine, Houston, TX 77030, USA; Solenic Medical, Inc., Addison, TX 75001, USA.
  • Prasad B; Solenic Medical, Inc., Addison, TX 75001, USA; Department of Radiology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
  • Kay W; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA.
  • Hunt C; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA.
  • Adams J; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA; Bone and Biofilm Research Lab, University of Utah, Salt Lake City, UT 84112, USA.
  • Kawaguchi B; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA; Bone and Biofilm Research Lab, University of Utah, Salt Lake City, UT 84112, USA.
  • Smith TB; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA.
  • Ashton N; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA; Bone and Biofilm Research Lab, University of Utah, Salt Lake City, UT 84112, USA.
  • Sadaphal V; Solenic Medical, Inc., Addison, TX 75001, USA; Department of Radiology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
  • Tepper J; Solenic Medical, Inc., Addison, TX 75001, USA.
  • Monogue M; Department of Internal Medicine, Infectious Diseases and Geographic Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA.
  • Ramirez JI; Department of Pathology, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
  • Jones OD; Department of Pathology, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
  • Shelton JM; Department of Internal Medicine, The University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
  • Evers BM; Department of Pathology, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
  • Serge R; StageBio, Frederick, MD 21701, USA.
  • Pybus C; Department of Pathology, University of Texas Southwestern Medical School, Dallas, TX 75390, USA.
  • Williams D; Department of Orthopaedics, University of Utah, Salt Lake City, UT 84112, USA; Bone and Biofilm Research Lab, University of Utah, Salt Lake City, UT 84112, USA.
  • Chopra R; Solenic Medical, Inc., Addison, TX 75001, USA; Department of Radiology, UT Southwestern Medical Center, Dallas, TX 75390, USA.
  • Greenberg DE; Department of Internal Medicine, Infectious Diseases and Geographic Medicine, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address: david.greenberg@utsouthwester
J Infect ; 89(5): 106271, 2024 Nov.
Article en En | MEDLINE | ID: mdl-39278276
ABSTRACT

OBJECTIVES:

We aimed to evaluate the effectiveness of alternating magnetic fields (AMF) combined with antibiotics in reducing Staphylococcus aureus biofilm on metal implants in a large animal model, compared to antibiotics alone.

METHODS:

Metal plates were inoculated with a clinical MRSA strain and then implanted into thirty-three ewes divided into three groups positive control, linezolid only, and a combination of linezolid and AMF. Animals had either titanium or cobalt-chrome plates and were sacrificed at 5 or 21 days post-implantation. Blood and tissue samples were collected at various time points post-AMF treatment.

RESULTS:

In vivo efficacy studies demonstrated significant biofilm reduction on titanium and cobalt-chrome implants with AMF-linezolid combination treatment compared to controls. Significant bacterial reductions were also observed in surrounding tissues and bones. Cytokine analysis showed improved inflammatory responses with combination therapy, and histopathology confirmed reduced inflammation, necrosis, and bacterial presence, especially at 5 days post-implantation.

CONCLUSIONS:

This study demonstrates that combining AMF with antibiotics significantly reduces biofilm-associated infections on metal implants in a large animal model. Numerical simulations confirmed targeted heating, and in vivo results showed substantial bacterial load reduction and reduced inflammatory response. These findings support the potential of AMF as a non-invasive treatment for prosthetic joint infections.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Infecciones Relacionadas con Prótesis / Biopelículas / Linezolid / Antibacterianos Límite: Animals Idioma: En Revista: J Infect Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Infecciones Estafilocócicas / Infecciones Relacionadas con Prótesis / Biopelículas / Linezolid / Antibacterianos Límite: Animals Idioma: En Revista: J Infect Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido