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Identification of Nontuberculous Mycobacterium Species by Polymerase Chain Reaction - Restriction Enzyme Analysis (PCR-REA) of rpoB gene in Clinical Isolates.
Yadav, Raj Narayan; Chowdary, Yellanki Yashwanth; Bhalla, Manpreet; Verma, Ajoy Kumar.
Afiliación
  • Yadav RN; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India.
  • Chowdary YY; Department of Medicine, Shri B. M. Patil Medical College Hospital and Research Center, BLDE (DU), Vijaypura, Karnataka, India.
  • Bhalla M; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India.
  • Verma AK; Department of Microbiology, National Institute of Tuberculosis and Respiratory Diseases, New Delhi, India.
Int J Mycobacteriol ; 13(3): 307-313, 2024 Jul 01.
Article en En | MEDLINE | ID: mdl-39277894
ABSTRACT

BACKGROUND:

Nontuberculous mycobacteria (NTM) infections are an emerging global health concern with increasing incidence. Conventional identification methods for NTM species in clinical settings are prone to errors. This study evaluates a newer method, polymerase chain reaction-restriction enzyme analysis (PCR-REA) of the rpoB gene, for NTM species identification. The study identified NTM species in clinical samples using conventional biochemical techniques and compared the results with PCR-REA of the rpoB gene. This cross-sectional study was conducted at a tertiary health-care center in North India over 18 months, analyzing both pulmonary and extrapulmonary samples.

METHODS:

Two hundred and forty-seven NTM isolates were identified using phenotypic and biochemical methods. The same isolates were subjected to rpoB gene amplification by PCR followed by REA using Msp I and Hae III enzymes.

RESULTS:

Conventional methods identified 12 different NTM species (153 slow-growing and 94 rapid-growing), whereas PCR-REA identified 16 species (140 slow-growing, 107 rapid-growing). The Mycobacterium avium intracellulare complex was the most common species isolated. PCR-REA demonstrated higher resolution in species identification, particularly in differentiating within species complexes.

CONCLUSIONS:

PCR-REA of the rpoB gene proves to be a simple, rapid, and more discriminative tool for NTM species identification compared to conventional methods. This technique could significantly improve the diagnosis and management of emerging NTM infections in clinical settings.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Micobacterias no Tuberculosas / Infecciones por Mycobacterium no Tuberculosas Límite: Female / Humans / Male País/Región como asunto: Asia Idioma: En Revista: Int J Mycobacteriol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Reacción en Cadena de la Polimerasa / Micobacterias no Tuberculosas / Infecciones por Mycobacterium no Tuberculosas Límite: Female / Humans / Male País/Región como asunto: Asia Idioma: En Revista: Int J Mycobacteriol Año: 2024 Tipo del documento: Article País de afiliación: India Pais de publicación: India