Your browser doesn't support javascript.
loading
Faecal microbiota and cytokine profiles of rural Cambodian infants linked to diet and diarrhoeal episodes.
Dalby, Matthew J; Kiu, Raymond; Serghiou, Iliana R; Miyazaki, Asuka; Acford-Palmer, Holly; Tung, Rathavy; Caim, Shabhonam; Phillips, Sarah; Kujawska, Magdalena; Matsui, Mitsuaki; Iwamoto, Azusa; Taking, Bunsreng; Cox, Sharon E; Hall, Lindsay J.
Afiliación
  • Dalby MJ; Microbes, Infection and Microbiomes, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK.
  • Kiu R; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Serghiou IR; Microbes, Infection and Microbiomes, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK.
  • Miyazaki A; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Acford-Palmer H; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Tung R; School of Tropical Medicine & Global Health, Nagasaki University, Nagasaki, Japan.
  • Caim S; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Phillips S; National Maternal and Child Health Centre, Ministry of Health, Phnom Penh, Cambodia.
  • Kujawska M; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Matsui M; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Iwamoto A; Microbes, Infection and Microbiomes, College of Medicine and Health, University of Birmingham, Birmingham, B15 2TT, UK.
  • Taking B; Food, Microbiome & Health, Quadram Institute Bioscience, Norwich Research Park, Norwich, NR4 7UQ, UK.
  • Cox SE; Intestinal Microbiome, School of Life Sciences, ZIEL - Institute for Food & Health, Technical University of Munich, Freising, 80333, Germany.
  • Hall LJ; School of Tropical Medicine & Global Health, Nagasaki University, Nagasaki, Japan.
NPJ Biofilms Microbiomes ; 10(1): 85, 2024 Sep 14.
Article en En | MEDLINE | ID: mdl-39277573
ABSTRACT
The gut microbiota of infants in low- to middle-income countries is underrepresented in microbiome research. This study explored the faecal microbiota composition and faecal cytokine profiles in a cohort of infants in a rural province of Cambodia and investigated the impact of sample storage conditions and infant environment on microbiota composition. Faecal samples collected at three time points from 32 infants were analysed for microbiota composition using 16S rRNA amplicon sequencing and concentrations of faecal cytokines. Faecal bacterial isolates were subjected to whole genome sequencing and genomic analysis. We compared the effects of two sample collection methods due to the challenges of faecal sample collection in a rural location. Storage of faecal samples in a DNA preservation solution preserved Bacteroides abundance. Microbiota analysis of preserved samples showed that Bifidobacterium was the most abundant genus with Bifidobacterium longum the most abundant species, with higher abundance in breast-fed infants. Most infants had detectable pathogenic taxa, with Shigella and Klebsiella more abundant in infants with recent diarrhoeal illness. Neither antibiotics nor infant growth were associated with gut microbiota composition. Genomic analysis of isolates showed gene clusters encoding the ability to digest human milk oligosaccharides in B. longum and B. breve isolates. Antibiotic-resistant genes were present in both potentially pathogenic species and in Bifidobacterium. Faecal concentrations of Interlukin-1alpha and vascular endothelial growth factor were higher in breast-fed infants. This study provides insights into an underrepresented population of rural Cambodian infants, showing pathogen exposure and breastfeeding impact gut microbiota composition and faecal immune profiles.
Asunto(s)
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Población Rural / Bifidobacterium / ARN Ribosómico 16S / Citocinas / Diarrea / Heces / Microbioma Gastrointestinal Límite: Female / Humans / Infant / Male País/Región como asunto: Asia Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Población Rural / Bifidobacterium / ARN Ribosómico 16S / Citocinas / Diarrea / Heces / Microbioma Gastrointestinal Límite: Female / Humans / Infant / Male País/Región como asunto: Asia Idioma: En Revista: NPJ Biofilms Microbiomes Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos