State-of-the-Art Metagenomic Sequencing and Its Role in the Diagnosis of Periprosthetic Joint Infections.

Fida, Madiha; Tande, Aaron J

Fida, Madiha; Tande, Aaron J.

Afiliación

Fida M; Division of Public Health, Infectious Diseases and Occupational Medicine, Department of Medicine, Mayo Clinic, Rochester, MN, USA. Electronic address: fida.madiha@mayo.edu.
Tande AJ; Division of Public Health, Infectious Diseases and Occupational Medicine, Department of Medicine, Mayo Clinic, Rochester, MN, USA.

Infect Dis Clin North Am ; 2024 Sep 13.

Article en En | MEDLINE | ID: mdl-39277504

ABSTRACT

ABSTRACT

Metagenomic next-generation sequencing (mNGS) is increasingly being recognized as a valuable diagnostic tool for periprosthetic joint infections (PJIs). This study reviews the diagnostic utility of mNGS, highlighting its improved sensitivity in detecting pathogens, particularly in culture-negative and polymicrobial infections. However, the clinical application of this method is hindered by challenges such as the prevalence of host DNA, the necessity for extensive bioinformatic analysis, and the potential for contamination, which can lead to misinterpretation of results. As mNGS continues to evolve, it holds significant potential to improve the management of PJI and enhance the application of precision medicine in orthopedic infections.

Palabras clave

Antimicrobial resistance genes; Metagenomics sequencing; NGS; Prosthetic joint infections; mNGS

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Infect Dis Clin North Am Asunto de la revista: DOENCAS TRANSMISSIVEIS Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

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